Novel Micropatterned Cardiac Cell Cultures with Realistic Ventricular Microstructure
نویسندگان
چکیده
منابع مشابه
Conduction block in micropatterned cardiomyocyte cultures replicating the structure of ventricular cross-sections.
AIMS Structural and functional heterogeneities in cardiac tissue have been implicated in conduction block and arrhythmogenesis. However, the propensity of specific sites within the heart to initiate conduction block has not been systematically explored. We utilized cardiomyocyte cultures replicating the realistic, magnetic resonance imaging-measured tissue boundaries and fibre directions of ven...
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Human embryonic stem (hES) cells are generally cultured on top of a feeder layer formed by mitotically inactivated murine embryonic fibroblasts (MEFs) to maintain their undifferentiated state. The culture of hES cells in this co-culture system presents several challenges since it is difficult to control cell cluster size. Large cell clusters tend to differentiate at the borders, and clusters wi...
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Micropatterned cellular co-cultures were fabricated using three major extracellular matrix components: hyaluronic acid (HA), fibronectin (FN) and collagen. To fabricate co-cultures with these components, HA was micropatterned on a glass substrate by capillary force lithography, and the regions of exposed glass were coated with FN to generate cell adhesive islands. Once the first cell type was i...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2009
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2009.02.019